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Novus Biologicals
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INF2 antibody was raised in Rabbit using Human INF2 as the immunogen. Rabbit polyclonal INF2 antibody.
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The INF2 Antibody [Biotin] from Novus is a INF2 antibody to INF2. This antibody reacts with Human. The INF2 antibody has been validated for the following applications: Western Blot, ELISA.
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The INF2 Antibody [FITC] from Novus is a INF2 antibody to INF2. This antibody reacts with Human. The INF2 antibody has been validated for the following applications: Western Blot, ELISA.
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The INF2 Antibody [Alexa Fluor® 488] from Novus is a INF2 antibody to INF2. This antibody reacts with Human. The INF2 antibody has been validated for the following applications: Western Blot, ELISA.
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INF2 Antibody raised in Rabbit validated in E, WB in Human.
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Image Search Results
Journal: Neurobiology of disease
Article Title: Ethanol-induced ceramide production causes neuronal apoptosis by increasing MCL-1S-mediated ER-mitochondria contacts.
doi: 10.1016/j.nbd.2023.106009
Figure Lengend Snippet: Fig. 4. IP3R-MCL-1S interaction promotes F-actin polymerization through CaM-mediated INF2 activation. A SH-SY5Y cells were incubated with EtOH for 24 h. ER fraction was mentioned in methods. MCL-1S was detected by western blot. Anti-Calnexin was used as ER marker. n = 5. B, C SH-SY5Y cells and iPSC-neurons were treated with EtOH for 24 h. IP3R was immunoprecipitated with anti-MCL-1S and anti-IP3R antibodies (left). The expression of MCL-1S, IP3R, and β-Actin in total cell lysates is shown (right). n = 5. D SH-SY5Y cells treated with EtOH for various time and then loaded with Mag-Fluo-4 (1 μM). The amount of ER calcium was measured by using flow cytometer. n = 5. E SH-SY5Y cells were transfected with MCL-1S siRNA or NT siRNA for 24h prior to EtOH exposure for 24 h. Mag-Fluo-4-positive cells were analyzed with flow cytometer. n = 5. F SH-SY5Y cells were immunostained with CaM antibody and ER. ER was stained by ER dye (red). Co-localization of CaM (green) and ER (red) was visualized with SRRF imaging system. Scale bars are 8μm. n = 5. G SH-SY5Y cells were transfected with INF2-CAAX siRNA or NT siRNA for 24h prior to EtOH exposure for 24 h. Mitochondrial fraction was mentioned in methods. F-actin was detected by western blot. Anti-TOMM20 was used as mitochondria marker. n = 5. H SH-SY5Y cells were immunostained with phalloidin and TOMM20 antibody. Co- localization of phalloidin (green) and TOMM20 (red) was visualized with SRRF imaging system. Scale bars are 5μm. n = 5. All blot, immunofluorescence, and flow cytometer images are representative. Quantitative data are shown as a mean ± S.E.M. * indicates p < 0.05 vs. control, # indicates p < 0.05 vs. EtOH. (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet:
Techniques: Activation Assay, Incubation, Western Blot, Marker, Immunoprecipitation, Expressing, Flow Cytometry, Transfection, Staining, Imaging, Immunofluorescence, Control